Mapping Neurons in Eyewire II
Once you have access to EyeWire II, use the Starburst Sheet to find cells that need to be mapped.
Instructions:
1. Choose a cell ID from the Proofreading tab
2. Click the START LINK
3. Copy in your SEGMENT ID and paste it into the START LINK
4. Go to the soma (cell body) and center your axes into a colored-in portion of the soma
5. Copy the coordinates (top of screen) and paste them into this spreadsheet. This should look like 3 comma-separated numbers
6. Begin proofreading. Use the annotation layers to mark your cell as you go along
7. Fill in this sheet as you go. Keep notes on in-progress cells, and update when complete.
**Important: Do NOT merge the nucleus to your cell! We also will not be merging in mitochondria.**
Legend
Soma ⚪️ The very first thing to do! Drop an annotation point into the soma. Copy its coordinates and paste them into this spreadsheet
True End Place an annotation point onto every branch end where the branch naturally terminates.
Can’t Fix If you believe the branch should continue but you are unable to locate the next segment, place this annotation point.
Hits Edge If the branch cannot be extended because it has reached the end of the dataset, place this annotation point.
Notes A place for your personal notes. You can use this to track branches you still need to check, or drop points for difficult spots you want to revisit.
+ If none of these annotation layers meet your needs, you can add your own!
CTRL+CLICK (or RIGHT CLICK) to add a new layer! Rename it something useful to you.
FAQ
1. Some IDs show only small fragments. You can still proofread these. Trace them out until you can see whether they are SACs or another type of cell.
2. If a cell is clearly not an SAC you can stop proofreading and mark it as such.
3. If you are unsure if the cell is an SAC or another type, you should proofread it to completion. Although we want to focus on proofreading SACs, there is no harm done in proofreading the occassional non-SAC.
Example proofread Starburst Cell from KrzysztofKruk: https://spelunker.cave-explorer.org/#!middleauth+https://global.daf-apis.com/nglstate/api/v1/6595401151938560
