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Hi everyone this is rprentki

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with another tutorial video for EyeWire

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This is going to be the last practice

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cube tutorial, so after this

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one you'll have graduated and you'll be playing EyeWire.

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Once again, you're not going to get any feedback

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While you're playing, you'll get a little bit afterwards

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and you'll get points

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So, we're still working with starburst

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amacrine neurons, which are

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long and thin, and tend to have a

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bouton, which is a blob-like structure

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that can be a little bit difficult to color

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let's get started

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Alright, so I'm just filling in these little pieces, here

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around the start point, and now

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We're going to continue tracing.

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oops, and I just put something outside of the line

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you can see that right there, it's clearly outside the line, so

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deselect that with a right click

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And, one of the reasons that

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a starburst amacrine cells

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can be difficult is because

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the borders are a little bit cloudy

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If I hit the shift key

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to get rid of the color, you can see

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on the  upper left part of my dark blue piece

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that there's just the

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very, very tiny hint

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of a border there, that's really

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difficult to see. so sometimes when

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you're adding stuff it's really beneficial to

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check in the 3D and make

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sure what you're working with seems like it's all part

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of the same cell, because it's possible

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you could pick up something that

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belongs to a different neuron

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okay, so I'm going to follow this

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piece down here

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alright, I'm just filling in a few more pieces as we go

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So you can see this blob right

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here is one of the boutons

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and it looks like this one actually

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has quite a few of them

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usually there will be one long thin

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branch, and then just

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one bouton in it, but we got lucky

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and got a couple

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it will also make this one slightly

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more challenging perhaps.

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Okay, so I'm going to follw this little one

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right here  as it breaks off

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the main one and see if it goes anywhere

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and remember, it's important to only follow one

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piece at a time, because otherwise you're

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liable to get lost

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alright, it keeps on getting smaller, and smaller

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let's see

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It can be really helpful to

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go back and forth sometimes

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it will make it easier for you to see what's there

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and what's not

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Okay, I'm going to hit the shift key to check this

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right up here. it looks like this might go

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let's take a look. and then I'm going to

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zoom in on the 3D

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just to make sure that looks okay

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alright, that's a nice smooth continuation

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there, that's got a nice round nub

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so that probably ends there

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and remember, if you see a

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jagged edge on something in the 3D, that's a

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really good indication that there's something missing

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so this one looks like it just disappears

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So we're going to scroll back up

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and continue tracing this piece

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and, we're very nearly done with this

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one branch now

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oops, okay, overshot it just a hair

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alright, we've hit the edge of the cube

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so now, what I'm going to do is

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move back down this a little bit

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and just look and see if there are any areas

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where I think I might have missed something

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and so what I see in the 3D is

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right here, see the little hole?

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so double click, and that will take me to that location

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In the 2D

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Okay, so I filled that in

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and then I'm also looking at the top of this bouton,

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of this blob thing right here

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and it looks like there's a little bit of a rough edge

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right here, so I'm going to 

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take a look at that as well

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okay, and I think that might be around here

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Ah, there we go

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and see, I would have missed this, had I not

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gone back and looked at the 3D

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and that little tell-tale rough edge told me

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that I had something missing

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So now, I'm going to look down here again

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this one, right here

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looks like there might be a hint of the rough edge

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on this dark blue one

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so we're going to look at it 

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Okay, so I think that's right here

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ah, okay, we did miss something

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would you look at that

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it's a nice big blob, and let's follow that

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to the edge and make sure we haven't

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missed anything else

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alright, one more look in the 3D

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ooh, over here it looks like there might be another

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jagged, so let's just double check that

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and that's over here, now

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in the 2D

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this little corner perhaps

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and it looks like it's about

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to...it's almost disappeared

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it might continue on 

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alright, so now I'm looking at this in the 3D

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and I'm thinking that it's probably not correct

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so this one right here

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that one does not look like it

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attaches to this one, which came out of our dark blue piece

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so let's deselect it

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and then let's see if this 

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little one continues at all, or if it disappears

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so it looks like it continues right there

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Okay, ah

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There we go, I just added

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another little blob, and this one looks

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like it fits on nicely

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See how it's got a smooth continuation?

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alright, and that disappears

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So remember

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We only found

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this last one and the one before

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Because we went back to double check in the 3D

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so that's why checking in the 3D is important

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And, we're done, congratulations

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you're ready to move on to playing EyeWire