1 00:00:00.0 --> 00:00:02.0 Hi everyone, this is rprentki 2 00:00:02.0 --> 00:00:04.0 with the second of the 3 00:00:04.0 --> 00:00:06.0 starburst amacrine cell tutorials 4 00:00:08.0 --> 00:00:10.0 there characterized by long thin branches 5 00:00:10.0 --> 00:00:12.0 and blobs (or boutons) 6 00:00:12.0 --> 00:00:14.0 and boutons tend to have 7 00:00:14.0 --> 00:00:16.0 several little nubs coming 8 00:00:16.0 --> 00:00:18.0 out of them, and one long 9 00:00:18.0 --> 00:00:20.0 branch that will continue on 10 00:00:20.0 --> 00:00:22.0 the nubs should have rounded 11 00:00:22.0 --> 00:00:24.0 edges, if there's a jagged edge 12 00:00:24.0 --> 00:00:26.0 of stuff coming off of the bouton 13 00:00:26.0 --> 00:00:28.0 then that usually means that 14 00:00:28.0 --> 00:00:30.0 there is something missing there 15 00:00:30.0 --> 00:00:32.0 Let's see if we can do this and I'll give you 16 00:00:32.0 --> 00:00:35.0 a better context to what I was just talking about 17 00:00:46.0 --> 00:00:48.0 A lot of little stuff missing here, let's see if we can 18 00:00:48.0 --> 00:00:50.0 fill that in a little bit better 19 00:00:56.0 --> 00:00:58.0 Alright, so this is the bouton, 20 00:00:58.0 --> 00:01:00.0 the blob that we were talking about 21 00:01:00.0 --> 00:01:02.0 a minute ago, and you'll note 22 00:01:02.0 --> 00:01:04.0 that there are 23 00:01:04.0 --> 00:01:07.5 several things right here 24 00:01:07.5 --> 00:01:09.5 that look like they are jagged 25 00:01:09.5 --> 00:01:11.5 so that means there's a bunch of stuff that's missing 26 00:01:11.5 --> 00:01:13.5 so let's start trying to fill that in 27 00:01:13.5 --> 00:01:15.0 and smooth it out 28 00:01:26.0 --> 00:01:28.5 There's a lot of stuff missing on the inside too 29 00:01:28.5 --> 00:01:30.0 but hopefully we'll get to start 30 00:01:30.5 --> 00:01:32.0 paying attention to the edges 31 00:01:36.0 --> 00:01:38.0 alright, so I can see 32 00:01:38.0 --> 00:01:40.0 up at the top of the blob 33 00:01:40.0 --> 00:01:42.0 this little piece 34 00:01:43.0 --> 00:01:45.0 okay, and I can just... 35 00:01:47.0 --> 00:01:49.0 there we go, looking good 36 00:01:50.0 --> 00:01:52.0 okay, so I can see that there are several 37 00:01:52.0 --> 00:01:54.0 other little jagged pieces 38 00:01:54.0 --> 00:01:56.0 so let's try to and find them 39 00:01:56.0 --> 00:01:58.0 now a really neat trick that we have 40 00:01:58.0 --> 00:02:01.0 is you can double click on something in the 3d 41 00:02:01.0 --> 00:02:03.0 so like that, and it will 42 00:02:03.0 --> 00:02:05.0 take you there in the 2D 43 00:02:05.0 --> 00:02:07.0 so you can inspect stuff more closely 44 00:02:07.0 --> 00:02:09.0 so let's explore this little point 45 00:02:09.0 --> 00:02:11.0 that I double clicked on 46 00:02:11.0 --> 00:02:13.0 and it looks jagged in the 3D, which 47 00:02:13.0 --> 00:02:15.0 indicates tot me that there's something missing 48 00:02:15.0 --> 00:02:18.0 so let's see what we can fill in 49 00:02:23.0 --> 00:02:25.0 And that disappears right there 50 00:02:25.0 --> 00:02:28.0 see how that looks much smoother up there now? 51 00:02:28.0 --> 00:02:30.0 it means we're doing our job 52 00:02:30.0 --> 00:02:32.0 let's go down a little bit and see 53 00:02:32.0 --> 00:02:33.5 if we can fill this in 54 00:02:33.5 --> 00:02:37.0 okay, so this one looks like it continues there 55 00:02:42.0 --> 00:02:44.0 alright so that one looks okay 56 00:02:44.0 --> 00:02:46.0 and it looks like there's one other little 57 00:02:46.0 --> 00:02:48.0 spot right here that may 58 00:02:49.0 --> 00:02:53.0 have something missing 59 00:02:53.0 --> 00:02:56.0 okay, and so I think this one is probably, oops 60 00:02:56.0 --> 00:02:58.0 thats me clicking on the wrong thing 61 00:02:58.0 --> 00:03:00.0 let's try that again 62 00:03:03.0 --> 00:03:05.0 hmm that looks a little fishy to me 63 00:03:10.0 --> 00:03:12.0 but let's carry on a little bit 64 00:03:12.0 --> 00:03:14.0 and just make sure 65 00:03:18.0 --> 00:03:21.0 yeah, so that's definitely not connected 66 00:03:21.0 --> 00:03:23.0 Let's deselect both of those 67 00:03:23.0 --> 00:03:26.0 alright, so... 68 00:03:26.0 --> 00:03:29.0 maybe there isn't actually something missing there 69 00:03:29.0 --> 00:03:31.0 it looks okay 70 00:03:31.0 --> 00:03:34.0 let's now try and find the branch that continues on 71 00:03:34.0 --> 00:03:36.0 so, this would be the last 72 00:03:36.0 --> 00:03:38.0 one that we haven't checkout out, right here 73 00:03:38.0 --> 00:03:40.0 and see how the edge is 74 00:03:40.0 --> 00:03:43.0 a little bit jagged. let's double click there 75 00:03:51.0 --> 00:03:53.0 hmm a little bit tricky 76 00:03:53.0 --> 00:03:55.0 ah there we go, so you're going to have to scroll back 77 00:03:55.0 --> 00:03:58.0 and forth a few times, sometimes to get that 78 00:03:58.0 --> 00:04:00.0 now see how this light blue one right here, looks 79 00:04:00.0 --> 00:04:02.0 almost like it's reaching out 80 00:04:02.0 --> 00:04:04.0 in the upper right direction 81 00:04:04.0 --> 00:04:06.0 So that's the way it's going 82 00:04:06.0 --> 00:04:08.0 So I think right there 83 00:04:08.0 --> 00:04:10.0 and let's take a look in the 3D 84 00:04:10.0 --> 00:04:12.5 to make sure, okay that looks okay 85 00:04:12.5 --> 00:04:14.0 let's carry on 86 00:04:20.0 --> 00:04:22.0 ah, there we go, if we zoom out 87 00:04:22.0 --> 00:04:24.0 a little bit in the 3D 88 00:04:25.0 --> 00:04:27.0 ah, much better 89 00:04:27.0 --> 00:04:29.0 so, we can see that it's 90 00:04:29.0 --> 00:04:31.5 almost going all the way across now 91 00:04:31.5 --> 00:04:33.5 Let's just finish that off 92 00:04:36.0 --> 00:04:39.0 and we're done, let's move on